Fig. 4

Knockdown of RHPN2 suppresses prostate cancer cell proliferation, invasion, and migration while accelerates apoptosis. a RHPN2 protein expression in cells after silencing of RHPN2 determined using western blot analysis; *, p < 0.05. b prostate cancer cell invasion and migration upon treatment with shRHPN2 plasmids detected using Transwell assay (200 ×); *, p < 0.05. c prostate cancer cell colony-forming ability upon treatment with shRHPN2 detected using clonogenic assay; *, p < 0.05. d RHPN2 protein expression in cells treated with upregulated miR-205 or upregulated RHPN2 detected using western blot analysis; e prostate cancer cell invasion and migration following treatment by upregulated miR-205 or upregulated RHPN2 detected using Transwell assay (200 ×); *, p < 0.05 vs. the control group. Measurement data were expressed as mean ± standard deviation; comparisons between two groups were analyzed using unpaired t-test; comparisons among multiple groups were analyzed by one-way analysis of variance; the experiment was repeated three times independently