Fig. 5

Characterization of hBMSCs-secreted exosomes. a, b morphological characteristics of hBMSCs at 24 h (× 400) and day 8 of culture (× 200); c characterization of the surface makers of hBMSCs by flow cytometry; d ultrastructure of exosomes observed under a transmission electron microscope (scale bar = 100 nm); e analysis of size and concentration of exosomes by nanoparticle tracking analyzer; f exosome surface markers (Hsp70 and CD63) and exosome negative marker CANX detected using western blot analysis; g miR-205 expression in hBMSCs and exosomes determined using RT-qPCR; *, p < 0.05. Measurement data were expressed as mean ± standard deviation; comparisons between two groups were analyzed using unpaired t-test; the experiment was repeated 3 times independently