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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Lysine demethylase 2 (KDM2B) regulates hippo pathway via MOB1 to promote pancreatic ductal adenocarcinoma (PDAC) progression

Fig. 3

MOB1 regulated Hippo pathway in PDAC cells. a, PANC-1 cells were transfected with pMOB1 or control vectors, and BxPC-3 cells were transfected with siMOB1–1(siMOB1) or control siRNAs. The Hippo pathway proteins were analyzed with western blot. b, pLATS1, pYAP and pTAZ proteins were tested in MOB1 restored and knockdown cells that were untreated or treated with okadaic acid (OA, 10 μM) for 30 min. Total LATS1 protein levels in each sample were adjusted to equality before electrophoresis. c, PANC-1 cells were transfected with pMOB1 or control vectors for 24 h. Cell immunofluorescence was used to detect YAP (red) and TAZ (green) in PANC-1 at low cell density (nuclei were stained with DAPI (blue)). d and e, the 8xGTIIC-Luciferase reporter which is the YAP/TAZ-responsive synthetic promoter driving luciferase reporter was transfected into PANC-1, AsPC-1, BxPC-3 and FG cells in triplicate with MOB1 restored expression or siMOB1 or control vectors for 24 h. The promoter activity was examined by a dual luciferase assay kit

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