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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Lysine demethylase 2 (KDM2B) regulates hippo pathway via MOB1 to promote pancreatic ductal adenocarcinoma (PDAC) progression

Fig. 5

KDM2B regulated Hippo pathway and promoted PDAC proliferation, migration and invasion via MOB1. a, PANC-1 cells transfected with KDM2B shRNAs for 48 h. Total cell lysis, cytosolic and nuclear proteins were extracted and the protein levels of KDM2B, MOB1, YAP and TAZ were measured using western blot analysis (α-Tublin was used for internal control of total cell lysis and cytoplasmic fractions, and Histone H1 was used for internal control of nuclear fractions). b, PANC-1 cells were transfected with shKDM2B or control vectors for 24 h. Cell immunofluorescence was used to detect YAP (red) in PANC-1 at low cell density (nuclei were stained with DAPI (blue)). c, PANC-1 and AsPC-1 cells were co-transfected with 0.2 μg of the 8xGTIIC-Luciferase reporter, control vector or shKDM2B or shKDM2B + siMOB1 for 24 h. Promoter activity was examined using a dual luciferase assay kit. PANC-1 and AsPC-1 cells were transfected with control vectors or shKDM2B or shKDM2B + siMOB1 for 24 h. d and e, colony formation assay was performed and numbers of colonies were counted 14 days after. f, g, h and i, the migration and invasion of PANC-1 and AsPC-1 cells were determined as described in Materials and Methods. Representative tumor cell migrated or invaded were photographed

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