Fig. 5

miR-200c-mediated regulation of NRP1 expression in OC cell lines. a) Schematic representation of the conserved target sites for miR-141/200a-3p (red) and miR-429/200bc-3p (green) in the 3′ UTR of human NRP1, and Targetscan prediction of miR-200c-3p binding site within the NRP1 3′-UTR. b) The expression pattern of miR-200c was assessed by qRT-PCR assays in OC tumor tissues from 28 patients and noncancerous ovarian tissues from 12 healthy donors. ***, p < 0.0005. c) Correlation analysis between NRP1 and miR-200c expression was performed in OC tumor tissues from 28 patients. d, e) UWB, UWB-BRCA and SKOV3 cells were analyzed at basal conditions (d) or after Olaparib treatment (e), and miR-200c expression was determined by qRT-PCR analysis. Each experiment was performed in triplicate, and miRNA levels were normalized to U6 expression. Error bars represent standard deviations. *, p < 0.05, **, p < 0.005, ***, p < 0.0005 vs. UWB cells (d) or vs control (DMSO) (e)