Fig. 6

Effect of miR-200c overexpression on NRP1. SKOV3 cells were stably transfected with a plasmid carrying the precursor of miR-200c (miR-200c) and its corresponding vector control (CTRL). miR-200c expression was assessed by qRT-PCR analysis (a). NRP1 protein (b) and mRNA (c) expression were evaluated by Western blot analysis and qRT-PCR, respectively. For Western blot analysis, tubulin expression was used as internal control, and the images are representative of at least three independent experiments. The intensity of the bands was evaluated by densitometric analysis, normalized and reported as relative expression with respect to control (CTRL). For qRT-PCR, each experiment was performed in triplicate. miRNA levels were normalized to U6 expression, while mRNA levels were normalized to GAPDH mRNA expression. Error bars represent standard deviations. **, p < 0.005 vs. CTRL