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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: MiR-200c sensitizes Olaparib-resistant ovarian cancer cells by targeting Neuropilin 1

Fig. 7

Effect of miR-200c overexpression on SKOV3 response to Olaparib. SKOV3 cells were stably transfected with a plasmid carrying the precursor of miR-200c (miR-200c) and its corresponding vector control (CTRL), then treated for 144 h with Olaparib. a, b) Cell viability and colony forming efficiency were determined by MTT assay and clonogenic assay, respectively. Mean values obtained from two independent experiments, each performed in triplicate, are reported in graph. Error bars represent standard deviations. ***, p < 0.0005 vs. CTRL cells or vs. control (DMSO). c) The presence of γH2AX foci (red) was assessed by immunofluorescence analysis. Nuclei (blue) were visualized with 4′, 6-diamidino-2-phenylindole (DAPI). Images were captured under ApoTome microscope at 40x magnification. Quantification of γH2AX foci was determined by measuring red fluorescence intensity with ImageJ software. Mean values obtained from measurements of five microscopic fields randomly taken from three independent experiments are reported in graph. Error bars represent standard deviations. **, p < 0.005 vs. control (DMSO)

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