Fig. 1

BRD7 inhibits cell migration and invasion in breast cancer cells. a qPCR and western blotting analysis of BRD7 expression in MDA231 and MCF7 cells stably transfected with BRD7 expression plasmid or control. Data represent means ± SDs. ***, p < 0.001. b CCK8 analysis of cell proliferation in MDA231 and MCF7 cells stably transfected with BRD7 expression plasmid or control. Data represent means ± SDs. ***, p < 0.001. c Scratch wound healing analysis of cell migration in MDA231 and MCF7 cells stably transfected with BRD7 expression plasmid or control. Quantification of wound recovery rate of the two group. Data represent means ± SEMs. *, p < 0.05, **, p < 0.01, ***, p < 0.001. d Matrigel invasion analysis of cell invasive capabilities in MDA231 and MCF7 cells stably transfected with BRD7 expression plasmid or control. Data represent means ± SEMs. *, p < 0.05. e Three-dimensional invasion analysis of cell invasive capabilities in MDA231 cells stably transfected with BRD7 expression plasmid or control. The white arrows represent prominent protrusions, scale bar, 50 μm. f Quantification of invasive and non-invasive clonal spheroids in BRD7 overexpression and control group. Data represent means ± SDs