Fig. 2

Silencing ITGA2 suppresses the aggressive ability of malignant cancer in vitro a and b. RT-PCR (a) and Western blot analysis (b) of ITGA2 expression in PANC-1, HepG2, SGC-7901, and MDA-MB-231 cells infected with sh-Control or sh-ITGA2s. GAPDH served as an internal reference. Data presented as the mean ± SD of three independent experiments. Each sh-ITGA2 group was compared with sh-Control group. Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. **, P < 0.01; ***, P < 0.001. c-e. PANC-1, HepG2, SGC-7901, and MDA-MB-231 cells were infected with sh-Control or sh-ITGA2 #1. The cells were harvested for colony formation assay (c), MTS assay (d), and migration assay (e) after forty-eight hours culturing. For e, representative images of migrated cells were shown based on a transwell assay. Each bar represents the mean ± SD of three independent experiments. Each sh-ITGA2 group was compared with sh-Control group. Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests.. **, P < 0.01; ***, P < 0.001