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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Overexpressed ITGA2 promotes malignant tumor aggression by up-regulating PD-L1 expression through the activation of the STAT3 signaling pathway

Fig. 3

Overpressed ITGA2 promotes the aggressive ability of malignant cancer in vitro. a and b. RT-PCR (a) and Western blot analysis (b) of ITGA2 expression in PANC-1, HepG2, SGC-7901, and MDA-MB-231 cells with normal or stably overexpressed ITGA2. GAPDH served as an internal reference. Data presented as the mean ± SD of three independent experiments. ITGA2 overexppression groups were compared with pcDNA 3.1 transfection group. Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. Compared groups were shown in the figures. ***, P < 0.001. c-e. PANC-1, HepG2, SGC-7901, and MDA-MB-231 cells were infected with pcDNA3.1 or ITGA2 plasmid. The cells were harvested for colony formation assay (c), MTS assay (d), and migration assay (e) after forty-eight hours culturing. For e, Representative images of migrated cells were shown based on a transwell assay. Each bar represents the mean ± SD of three independent experiments. ITGA2 overexppression groups were compared with pcDNA 3.1 transfection group. Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. Compared groups were shown in the figures. **, P < 0.01; ***, P < 0.001

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