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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Overexpressed ITGA2 promotes malignant tumor aggression by up-regulating PD-L1 expression through the activation of the STAT3 signaling pathway

Fig. 5

ITGA2 transcriptionally increases PD-L1 expression in solid cancer cells. a and b. Volcano plot (a) and heatmap (b) to show the differential expressed genes of PANC-1 cells infected by si-Control or si-ITGA2. The blue points represented the downregulated genes, while red points represented the upregulated genes. c. Heatmap to show a subset of ITGA2 knockdown regulated genes participated in PD-L1 expression and PD-1 checkpoint pathway of PANC-1 cells. d-f. Forty-eight hours postinfection, PANC-1, HepG2, SGC-7901, and MDA-MB-231 cells infected with sh-Control or sh-ITGA2s were harvested for RT-PCR analysis (d), Western blotting analysis (e), and FACS analysis (f). Data are shown as means ± SD (n = 3). Each sh-ITGA2 group was compared with sh-Control group. Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. *, P < 0.05; **, P < 0.01; ***, P < 0.001. g-h. Forty-eight hours postinfection, PANC-1, HepG2, SGC-7901, and MDA-MB-231 cells infected with pcDNA3.1 or ITGA2 plasmids were harvested for RT-PCR analysis (g) and Western blotting analysis (h). Data are shown as means ± SD (n = 3–5). ITGA2 overexppression groups were compared with pcDNA 3.1 transfection group. Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. Compared groups were shown in the figures. **, P < 0.01; ***, P < 0.001

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