Fig. 8

ITGA2 increases PD-L1 expression via up-regulating the phosphorylation of STAT3 in cancer cells. a. MTS assay was carried out to measure the cell viability of PANC-1 cells infected with sh-Control or sh-ITGA2 #1 and treated with different chemicals. IC50 was shown as indicated. Heatmap to show the normalized IC50 ratio (log2[IC50ratio]). b. Forty-eight hours postinfection, PANC-1, HepG2, SGC-7901, and MDA-MB-231 cells infected with sh-Control or sh-ITGA2 were harvested for RT-PCR analysis. All data were shown as mean ± SD (n = 3). Each sh-ITGA2 group was compared with sh-Control group. Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. ns, not significant. c. Western blot analysis for IP samples of PANC-1, HepG2, SGC-7901, and MDA-MB-231 cells. d. Forty-eight hours postinfection, PANC-1, HepG2, SGC-7901, and MDA-MB-231 cells infected with sh-Control or sh-ITGA2 were harvested for Western blotting analysis. e and f. Forty-eight hours postinfection, PANC-1, HepG2, SGC-7901, and MDA-MB-231 cells infected with or without ITGA2 plasmids (e) and treated with or without STAT3 inhibitor (f) were harvested for Western blotting analysis.