Fig. 3

Effects of CPP on TRAIL receptors (DR4 and DR5). a. Immunohistochemical analysis of the expression of DR4 and DR5 in 50 cases of ESCC specimens and 33 cases of adjacent tissues of tumor. Scale bar, 50 μm. b. Immunohistochemical analysis of the percentage of DR4 and DR5 positive tissues in 50 cases of ESCC specimens. c. Western blot analysis of DR4 and DR5 protein levels in nine ESCC cells. d. YES-2, KYSE-150 and KYSE-510 cells were treated with various concentrations of CPP alone or in combination with TRAIL for 24 h. Changes in the expression levels of DR4 and DR5 proteins were examined by western blot. e-f. YES-2, KYSE-150 and KYSE-510 cells were treated with various concentrations of CPP for 24 h. Changes in the expression levels of DR4 and DR5 on the cell surface were analyzed by flow cytometry. g-h. YES-2, KYSE-150 and KYSE-510 cells were treated with various concentrations of CPP for 24 h. The expression of DR4 and DR5 mRNA were determined by qRT-PCR. Data are shown as the mean ± SEM. *P < 0.05, **P < 0.0.1