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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Combination of the natural compound Periplocin and TRAIL induce esophageal squamous cell carcinoma apoptosis in vitro and in vivo: Implication in anticancer therapy

Fig. 5

Binding of FoxP3 to the promoter region of DR4 and DR5 genes. a-b. Predicted binding sequence for transcription factor FoxP3 in DR4 (TNFRSF10A) and DR5 (TNFRSF10B). c-d. YES-2 and KYSE-150 cells were treated with various concentrations of CPP for 24 h. Then, cells were subjected to ChIP analysis with the FoxP3 antibody. The ChIP-enriched DNAs were amplified by PCR with primers specific for the binding region (BR) of FoxP3 with the DR4 or DR5 promoter. A non-binding region (NBR) was used as a negative control. e-f. A schematic diagram of constructed luciferase reporter genes. Luciferase reporter gene assays were conducted with contain wild FoxP3 binding site (#1 and #a), mutant FoxP3 binding site (#2 and #b) and non-containing FoxP3 binding site (#3 and #c) promoters of the DR4 and DR5 genes. g. Luciferase reporter assay was carried out with constructs of #1, #2, #3 in HEK293T cells and effect of CPP treatment on the activity of promoter of DR4 gene. h. Luciferase reporter assay was carried out with constructs of #a, #b, #c in HEK293T cells and effect of CPP treatment on the activity of promoter of DR5 gene

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