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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Mortaparib, a novel dual inhibitor of mortalin and PARP1, is a potential drug candidate for ovarian and cervical cancers

Fig. 2

Mortaparib-treated HeLa cells undergo apoptosis. Flow cytometric analysis showing increase in apoptotic cells in dose dependent manner following Mortaprib treatment (a). Western blot analysis showing molecular markers of apoptosis in control and Mortaparib-treated cells (b). Decrease in pro-caspase, BCl2, COX I, COX IV, Caspase-7, − 9, − 10 in Mortaparib-treated cells was recorded. Cleaved caspase-3 showed increase. Immunostaining showed decrease in COX I, COX IV, Procaspase 3 and BCl2 proteins in Mortaparib-treated cells (Scale bar = 20 μM). c. Caspase-3 activity, as evaluated using a fluoremetric assay, showed increase in dose-dependent manner (d). The quantitative data represents mean ± SD obtained from, at least, three independent experiments; p-values were calculated using Student’s t-test. * < 0.05, ** < 0.01 and *** < 0.001 represent significant, very significant and very very significant, respectively

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