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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: De-regulated STAT5A/miR-202-5p/USP15/Caspase-6 regulatory axis suppresses CML cell apoptosis and contributes to Imatinib resistance

Fig. 1

USP15 expression is significantly downregulated in CML. (a) qRT-PCR detected USP15 mRNA level in PBMCs of CML-CP patients (n = 30) and PBMCs of healthy donors (n = 30). Data are showed as mean ± ST from three independent experiments. Normalized to β-actin. **P < 0.01 vs. normal. (b) Western blot analysis was used to measure USP15 protein level in PBMCs of CML-CP patients (n = 30) and PBMCs of healthy donors (n = 30). The representative experiments were present. (c) Immunofluorescence analyzed the USP15 protein level and localization of USP15 in PBMCs of CML-CP patients and PBMCs of healthy donors. The representative results were shown. Scale bar = 64 μm. (d) qRT-PCR detected USP15 mRNA level in CML cell lines (K562 and KCL22) and PBMCs of healthy donors. ** P < 0.01 vs. normal. (e) Western blot analysis was used to measure USP15 protein level in CML cell lines (K562 and KCL22) and PBMCs of healthy donors. (f) Immunofluorescence stains analyzed the USP15 protein level and localization in CML cell lines (K562 and KCL22) compared with PBMCs of healthy donors. Scale bar = 64 μm. (g) K562 cells were transfected with USP15 overexpression vector pcDNA3.1-USP15 or empty vector and then cultured in different times. CCK-8 assay was used to test the cell viability. **P < 0.01, ***P < 0.001 vs pcDNA3.1-vector. (h) K562 cells were transfected with pcDNA4.1-USP15 or empty vector for 48 h. Cell apoptosis was detected by Annexin V-FITC/PI staining. Right panel shows the apoptosis rate from three independent experiments. **P < 0.01 vs. pcDNA3.1-vector

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