Fig. 2

Higher expression of YAP promotes cellular growth, migration, invasion and EMT. a The expression of YAP was higher in NSCLC cell lines compared with their control cell line, HBEC, analyzed by RT-PCR, western blot and qRT-PCR assays. b The protein level of nuclear YAP was higher in high metastasis lung cancer cell line 95-D than its control cell HBEC by the immunofluorescent staining assay. c Immunoblotting with densitometric quantitation demonstrating increased nuclear Yap and decreased p-YAP in 95-D cells than its normal cell line meanwhile YAP’s target genes, CTGF and Cyr61, were higher in 95-D cells. d-h A549 cells were transfected with siYAP or Myc-YAP, respectively. d The expression of YAP was analyzed by RT-PCR, Western blot and qPCR assays. e The cellular viability was analyzed by CCK8 assay. f Cellular migration growth was analyzed by scratch assay. g The cellular invasion growth was analyzed by transwell assay. h The expressions of E-cadherin and Vimentin were analyzed by RT-PCR, western blot assays. i The expression of YAP was higher in human lung cancer tissues compared with their normal adjacent lung tissues analyzed by RT-PCR, western blot and qRT-PCR assays (n = 8). j Immunohistochemical (IHC) assay of the expression of YAP and Snail in the human lung cancer tissues and their normal adjacent lung tissues (n = 15). k YAP was higher in nucleus from lung tumor tissues than their normal adjacent lung tissues (n = 15). l Kaplan Meier overall survival (OS) curves of YAP (p = 0.023 by log-rank test for significance) for human lung cancers. Results were presented as mean ± SD, and the error bars represent the SD of three independent experiments. *P < 0.05 or **P < 0.01 indicates a significant difference between the indicated groups (two-tailed, unpaired Student’s t-test or one-way analysis of variance)