Fig. 7

YAP promotes cell migration, invasion and EMT by regulating snail. a IHC assay of the expression of YAP and Snail in the human lung cancer tissues from pro-metastasis (Pro-M) and after-metastasis (After-M) (n = 10). b IHC assay of the expression of YAP and Snail from the tumor in situ (TIS) and tumor in metastasis (TIM) of lung cancers (n = 10). c, d The expression of Snail was detected in the HBEC and 95-D cells by RT-PCR, western blot, qPCR (c) and immunofluorescent staining (d) assays. e-g HBEC cells were transfected with snail or 95-D cells were transfected with si-snail, respectively. The expression of Snail was analyzed by RT-PCR, qPCR and western blot assays (e). The cellular viability was analyzed by CCK8 assay (f). The cellular migration growth was analyzed by scratch assay (g). h-p HBEC cells were transfected with YAP or co-transfected with YAP and si-snail, meanwhile 95-D cells were transfected with siYAP or co-transfected with siYAP and snail, respectively. The expressions of YAP and Snail were analyzed by RT-PCR, western blot and qPCR in HEBC and 95-D cells (h, i). The cellular viability was analyzed by CCK8 assay in HEBC (j) and 95-D cells (k). The cellular migration growth was analyzed by scratch assay in HBEC cells (l). The cell migration and invasion were analyzed by transwell assay in HBEC cells (m). The expression of Annexin V was analyzed by immunofluorescent staining assays in 95-D cells (n). The expressions of Vimentin and E-cadherin were analyzed by RT-PCR, western blot and qPCR in HEBC and 95-D cells (o, p). Results were presented as mean ± SD, and the error bars represent the SD of three independent experiments. *P < 0.05 or **P < 0.01 indicates a significant difference between the indicated groups (two-tailed, unpaired Student’s t-test or one-way analysis of variance)