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Fig. 9 | Journal of Experimental & Clinical Cancer Research

Fig. 9

From: Metformin-repressed miR-381-YAP-snail axis activity disrupts NSCLC growth and metastasis

Fig. 9

miR-381 inhibits lung cancer growth and metastasis in vivo by regulating snail via YAP. a RT-PCR, western blot, and qPCR analysis of the protein and mRNA levels of miR-381 and YAP in the A549 cells with stable expressions of controls (controlshNC), miR-381 inhibitors (miR-381 inhibitorsshNC) or co- expressions of miR-381 inhibitors and shYAP (miR-381 inhibitorsshYAP). b, c Xenografted A549 cell tumors with stably expressing controlshNC, miR-381 inhibitorsshNC and miR-381 inhibitorsshYAP in mice and the weights (b) and sizes (c) measured at regular intervals. d Kaplan-Meier overall survival (OS) curves of mice injected A549 cells with stably expressing controlshNC, miR-381 inhibitorsshNC and miR-381 inhibitorsshYAP. e, f The expressions of Ki67, Cleaved Caspase3, YAP, Snail, E-cadherin and Vimentin were analyzed by immunohistochemistry (e), RT-PCR, western blot and qPCR (f) assays from tumor tissues injected A549 cells with stably expressing controlshNC, miR-381 inhibitorsshNC and miR-381 inhibitorsshYAP in mice (n = 5). g Representative H&E stained microscopic images of the lung tumors originated from xenografted A549 cells with stably expressing controlshNC, miR-381 inhibitorsshNC and miR-381 inhibitorsshYAP in mice by subcutaneous injection. h The mRNA levels of miR-381, Ki67, YAP, Snail, E-cadherin and Vimentin were analyzed by were qPCR in the lung tumors originated from xenografted A549 cells with stably expressing controlshNC, miR-381 inhibitorsshNC and miR-381 inhibitorsshYAP in mice by subcutaneous injection. Results were presented as mean ± SD, and the error bars represent the SD of three independent experiments. i The diagram of metformin-repressed miR-381-YAP-snail axis activity disrupts NSCLC growth and metastasis. **P < 0.01 indicates a significant difference between the indicated groups (two-tailed, unpaired Student’s t-test or one-way analysis of variance)

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