Fig. 4From: ROCK2 deprivation leads to the inhibition of tumor growth and metastatic potential in osteosarcoma cells through the modulation of YAP activityVerteporfin inhibits YAP expression and activity as well as ROCK2 expression. a Western blot analysis of YAP in untreated (CTRL) or verteporfin-treated U-2OS cells. GAPDH was used as a loading control. b Dose- and time-dependent qPCR analysis of the expression of CYR61, CTGF and CCND1, conventional YAP downstream targets, in U-2OS parental cells. Data are shown as 2-ΔΔCt. Data are presented as the mean ± SE of three separate experiments (* p < 0.05, ** p < 0.01, *** p < 0.001, Student’s t test). GAPDH was used as a housekeeping gene. c Western blot analysis of ROCK2 in untreated (CTRL) or verteporfin-treated U-2OS cells. GAPDH was used as a loading control. d Expression of ROCK2 by immunofluorescence in U-2OS cells treated with verteporfin for 24–48 h. Digital images were taken under identical conditions using the image analysis software NIS-Elements (Nikon Italia). Scale bar, 20 μmBack to article page