Fig. 5

Verteporfin reduces the aggressiveness of OS cells. a Effects of verteporfin on the growth of U-2OS cells in anchorage-independent conditions. Each column (right) represents the mean ± SE of three separate experiments. Colonies were counted after 10 days. *** P < 0.0001, paired Student’s t test. Representative images (left) of spheroid colonies are shown. Scale bar, 200 μm b Effects of verteporfin on the migration of U-2OS cells in Transwell chambers. Each column represents the mean ± SE of three separate experiments. *** P < 0.0001, paired Student’s t test. c Effects of verteporfin after wound-healing assays scale bar, 100 μm d Immunofluorescence staining of N-cadherin and β-catenin in U-2OS cells after 24 h of treatment with verteporfin. Digital images were taken in identical conditions using the image analysis software NIS-Elements; scale bar, 20 μm. e Effects of verteporfin on the growth of PDX-OS#16-C2 in anchorage-independent conditions (upper) and on migration of these cells (lower). Each column represents the mean + SE of three separate experiments. *** P < 0.0001, paired Student’s t test