Fig. 2

miR-93-5p targets and negatively regulates FOXA1. a Predicted results of miRNAs that regulated FOXA1 in TargetScan, EVmiRNA, mirDIP, and microRNA databases. b miR-93-5p expression in CAFs-exo determined by RT-qPCR, normalized to GAPDH. c Prediction binding sites between miR-93-5p and FOXA1. d Luciferase activity of FOXA1-wt and FOXA1-mut in the presence of miR-93-5p mimic or mimic NC detected using dual-luciferase reporter gene assay. e miR-93-5p expression in CRC tissues (n = 75) and adjacent normal tissues (n = 75) determined by RT-qPCR, normalized to GAPDH. f Correlation between miR-93-5p and FOXA1 analyzed by Pearson. Values obtained from three independent experiments in triplicate are expressed as mean ± SD and analyzed by paired t test between CRC tissues and adjacent normal tissues and by unpaired t test between two groups. ^*p < 0.05 compared with adjacent normal tissues, or SW480 cells treated with mimic-NC plasmids