Fig. 4

CAFs enhance chemoresistance of CRC cells by delivering miR-93-5p via exosomes. The exosomes were isolated from CAFs overexpressing miR-93-5. Next, SW480 cells were co-cultured with NC-exo and miR-93-5-exo and exposed to irradiation. a miR-93-5p expression in CAFs-exo determined by RT-qPCR, normalized to GAPDH. b Cell viability in SW480 cells detected using CCK-8 assay. c–d Colony formation ability of SW480 cells detected using colony formation assay. e–f Cell cycle distribution in SW480 cells detected using flow cytometry. g–h Apoptosis of SW480 cells detected using flow cytometry. Values obtained from three independent experiments in triplicate are expressed as mean ± SD and analyzed by one-way ANOVA followed by Tukey’s post hoc test among multiple groups, and by repeated measures ANOVA followed by Bonferroni at different time points. ^*p < 0.05 compared with SW480 cells co-cultured with NC-exo; ^#p < 0.05 compared with non-irradiated cells