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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Exosome-mediated transfer of miR-93-5p from cancer-associated fibroblasts confer radioresistance in colorectal cancer cells by downregulating FOXA1 and upregulating TGFB3

Fig. 7

CAFs carrying exosomal miR-93-5p promote chemoresistance of CRC cells in vivo by regulating FOXA1. Before animal experiments, SW480 cells were co-cultured with agomir-NC-exo and agomir-93-5p-exo, and nude mice were subcutaneously injected with these cells, and subjected to irradiation. a Quantitative analysis for tumor volume in nude mice. b Representative tumor images in nude mice. c Quantitative analysis for tumor weight in nude mice. de Positive expression of Bax and Bcl2 proteins determined by immunohistochemistry (400 ×). (F) miR-93-5p expression and FOXA1 mRNA expression in xenografted tumors determined by RT-qPCR, normalized to GAPDH. gh FOXA1 protein bands and expression in xenografted tumors detected by Western blot analysis, normalized to GAPDH. Values obtained from three independent experiments in triplicate are expressed as mean ± SD and analyzed by one-way ANOVA followed by Tukey’s post hoc test among multiple groups, and repeated measures ANOVA followed by Bonferroni at different time points. n = 10. *p < 0.05 nude mice injected with SW480 cells without treatment; #p < 0.05 nude mice injected with SW480 cells transduced with agomir-NC-exo

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