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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Musashi2 contributes to the maintenance of CD44v6+ liver cancer stem cells via notch1 signaling pathway

Fig. 2

CD44v6+ HCC cells possessed characteristics of cancer stem cells. a Representative images of spheres and histogram analysis in indicated cells. CD44v6+ SNU-398 cells and MHCC-97 h cells processed enhanced self-renewal property than CD44v6- cells. Scale bar, 200 μm. b and c Representative images of transwell migration and invasion in indicated cells. Transwell migration and invasion assays showed that CD44v6+ SNU-398 cells and MHCC-97 h cells displayed higher migratory and invasion capacity than CD44v6- cells. Scale bar, 200 μm. d Representative images of colony formation assays in indicated cells. Colony formation assays demonstrated that CD44v6+ SNU-398 cells and MHCC-97 h cells exhibited higher proliferation and colony formation ability. e CD44v6+ and CD44v6- SNU-398 cells and MHCC-97 h cells were treated with Sorafenib for 24 h and evaluated by CCK8 toxic assay. It showed that CD44v6+ cells were more resistant to Sorafenib than CD44v6- cells. f The expression of cancer stemness-related genes, including Nanog, Oct4 and Sox2 in CD44v6+ and CD44v6- SNU-398 cells. β-actin was used as a normalized control. It showed that elevated stemness-related genes expressed in CD44v6+ SNU-398 cells than CD44v6- cells. g Efficiency of tumor formation of CD44v6+ cells and CD44v6- cells isolated from SNU-398 cell line. Number of injected cells: 1 × 105, 1 × 104, 1 × 103. n = 12. For statistical analysis, * p < 0.05, **p < 0.01 and ***p < 0.001, t test

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