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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Musashi2 contributes to the maintenance of CD44v6+ liver cancer stem cells via notch1 signaling pathway

Fig. 5

MSI2 maintained the stemness properties of CD44v6+ LCSCs via activating Notch1 signaling pathway. a External dataset from starBase v3.0 project with 374 HCC samples and 50 normal samples was used to analyze the correlation of MSI2 and Notch1. The results showed that MSI2 was positively related to Notch1 in clinical-pathology (r = 0.458, p = 8.02e-21). b Immunofluorescence images of MSI2 (red) and Notch1 (green) in HCC tissues. MSI2 was co-localized with Notch1 in HCC tissues. Scale bar, 50 μm. c Immunofluorescence images of CD44v6+ SNU-398 cells and CD44v6- cells for localization of MSI2 (red) and Notch1 (green). Histogram analysis of the relative fluorescence intensity of MSI2 and Notch1 in CD44v6+ cells and CD44v6- cells. Scale bar, 50 μm. d. Silencing MSI2 decreased the expression of Notch1 receptor and Notch1 pathway target genes in CD44v6+ SNU-398 cells. β-actin was used as a normalized control. e Western blot showed that overexpression of MSI2 increased the expression of Notch1 receptor and Notch1 pathway target genes in CD44v6- SNU-398 cells. β-actin was used as a normalized control

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