Fig. 7

MSI2 bound to LFNG mRNA and protein directly. a Schematic representation of MSI2 molecular interaction domains for interaction with RNA and with proteins. RRM, RNA recognition motif; PPD, protein-protein binding domain. b CD44v6+ cells and CD44v6- cells were isolated from SNU-398 cells. CD44v6+ cells were transfected with MSI2 shRNA1 or corresponding control virus, CD44v6- cells were transfected with Lv MSI2 or corresponding control virus. Lysates were precipitated with anti-MSI2 antibody and then immunoblotted (WB) for LFNG. Protein expression of MSI2 and LFNG was also analyzed. c CD44v6+ cells were transfected with MSI2 shRNA1 or corresponding control virus, CD44v6- cells were transfected with Lv MSI2 or corresponding control virus. RIP assays using anti-MSI2 antibody showed that MSI2 interacted with LFNG. The results of agarose electrophoresis of the PCR products were shown. d Schematic illustration the mechanism by which MSI2 activating Notch1 signaling pathway by binding to LFNG mRNA and protein directly