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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Correction to: The RNA binding protein RBMS3 inhibits the metastasis of breast cancer by regulating Twist1 expression

Fig. 2

RBMS3 inhibited the migration invasion of breast cancer cells in vitro and in vivo. a, b SUM-1315 and MDA-MB-231 cell lines were respectively transfected with lentivirus to overexpress (RBMS3) or knocked down (sh-1, sh-2, sh-3) RBMS3 expression. Western blot and qRT-PCR were applied to verify transfection efficiency. c-j RBMS3 inhibited the invasion and migration of breast cancer cells. c, g, e, i Transwell experiment was used to detect the invasion and migration ability of SUM-1315 cells. The lower panel of each picture showed migrating and invading numbers of SUM-1315 cells. Transwell assay performed in MDA-MB-231 cells were analyzed as in Fig. 2d, h, f, and j. Scale bars, 50 μm. (Reviewer #1 comment 5) Data were shown as mean ± SEM, *P < 0.05. k RBMS3 inhibited the lung metastasis in breast cancer cells. Representative bioluminescence images of the mice and HE staining of lung section showed the sizes and numbers of lung colonization in the RBMS3-overexpressed group and the control group, respectively. Metastasis nodules plot was generated by the H&E-stained lung sections of nude mice (n = 9). Data were shown as mean ± SEM, *P < 0.05, **P<0.001 (Reviewer #1 comment 6). SCR = Scrambled control; Sh = Short hairpin; NC = Negative control

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