Fig. 1

LINC00662 was highly expression in colon cancer tissues and cell lines and closely connected with OS and cell viability (a) RT-PCR assay was used to detected LINC00662 mRNA level in colon cancer tissues; (b) Kaplan-Meier analysis was used to showed survival rate of colon cancer patients with overexpression of LINC00662 and inhibition of LINC00662; (c) RT-PCR assay was used to detect LINC00662 mRNA level in colon cancer cell lines; (d) RT-PCR assay was used to detectLINC00662 mRNA level in LINC00662 overexpression plasmids transfected HCT29 and LS174T cells; (e) RT-PCR assay was used to detect LINC00662 mRNA level in LINC00662 knockdown plasmids transfected LOVO and CT26 cells; (f and g) CCK8 assay was used to detect cell viability of LINC00662 overexpression plasmids transfected HCT29 and LS174T cells; (h and i) CCK8 assay was used to detect cell viability of LINC00662 overexpression plasmids transfected LOVO and CT26 cells. GAPDH was used as a load control. Data are presented as the mean ± standard deviation. *P < 0.01 vs. normal group/ NCM460/pcDNA3.1-vector group/NC-siRNA group