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Fig. 10 | Journal of Experimental & Clinical Cancer Research

Fig. 10

From: LncRNA LINC00662 promotes colon cancer tumor growth and metastasis by competitively binding with miR-340-5p to regulate CLDN8/IL22 co-expression and activating ERK signaling pathway

Fig. 10

LINC00662 regulated the activation of ERK signaling pathway by targeting CLDN8 and IL22 (a) After LINC00662 knockdown plasmids and CLDN8/or IL22 overexpression plasmids were transfected into HCT29 cell, western blot assay was used to detect the expressions of CLDN8, IL22, p-ERK and ERK in protein levels; (b) After LINC00662 knockdown plasmids and CLDN8/or IL22 overexpression plasmids were transfected into CT26 cell, western blot assay was used to detect the expressions of CLDN8, IL22, p-ERK and ERK in protein levels; (c) After LINC00662 knockdown plasmids and CLDN8/or IL22 overexpression plasmids were transfected into HCT29 cell, western blot assay was used to detect the expressions of Bax, Bcl-2, XIAP, VEGF, MMP-2, E-cadherin and N-cadherin in protein levels; (d) After LINC00662 knockdown plasmids and CLDN8/or IL22 overexpression plasmids were transfected into CT26 cell, western blot assay was used to detect the expressions of Bax, Bcl-2, XIAP, VEGF, MMP-2, E-cadherin and N-cadherin in protein levels. GAPDH was used as a load control. Data are presented as the mean ± standard deviation. *P < 0.01 vs. NC-siRNA group, #P < 0.01 vs. NC1 group, ^P < 0.01 vs. NC2 group and &P < 0.01 vs. LINC00662-siRNA group

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