Fig. 9

LINC00662 regulated the proliferation, apoptosis, invasion and migration of colon cancer cells by targeting CLDN8 and IL22 (a) Western blot and RT-PCR assays were used to detect CLDN8 expression in protein and mRNA levels in CLDN8 overexpression plasmids transfected HCT29 and CT26 cells; (b) Western blot and RT-PCR assays were used to detect IL22 expression in protein and mRNA levels in IL22 overexpression plasmids transfected HCT29 and CT26 cells; After LINC00662 knockdown plasmids and CLDN8/or IL22 overexpression plasmids were transfected into HCT29 and CT26 cell, (c and d) CCK8 assay was used to detect cell viability, (e) Clone formation assay was used to detect cell proliferation, (f) Flow cytometry assay was used to detect cell apoptosis, (g) Transwell assay was used to detect cell invasion, (h) Wound healing assay was used to detect cell migration. GAPDH was used as a load control. Data are presented as the mean ± standard deviation. *P < 0.01 vs. NC-siRNA group, #P < 0.01 vs. NC1 group, ^P < 0.01 vs. NC2 group and &P < 0.01 vs. LINC00662-siRNA group