Fig. 3

DYNLT1 is a direct downstream miR-15b-3p target in GC cells. a miR-15b-3p target gene prediction using four bioinformatics tools (miRDB, RNA22, TarBase and TargetScan). b DYNLT1 mRNA levels in GC tissues and paired adjacent non-GC tissues (n = 108) analyzed using qRT-PCR. c GC tissue DYNLT1 expression was found to be significantly decreased, based on the TCGA database. d-f Western blotting and IHC analysis of DYNLT1 protein levels in GC tissues and adjacent non-GC tissues. Scale bar, 200 μm. g miR-15b-3p and DYNLT1 expression level association analysis using the 108 GC tissues. h-i Immunoblotting assays and qRT-PCR on DYNLT1 expression of miR-15b-3p inhibitor/inhibitor-NC/mimics/NC transfected BGC-823 and SGC-7901 cells. j DYNLT1 binding site of the wild-type (WT) and mutated type with miR-15b-3p. k Direct recognition of DYNLT1 3′-UTR by miR-15b-3p. Co-transfection of BGC-823 and SGC-7901 cells with WT or Mutant DYNLT1 3′-UTR and miR-15b-3p mimics, inhibitor or their corresponding normal control (NC or inhibitor-NC). The relative luciferase activity of BGC-823 and SGC-7901 cells were determined. Mean ± SEM of the results are presented