Fig. 1

The p38alpha MAPK pharmacological inhibition by SB203580 treatment hinders response to 5-FU. The HT29-sh/scr and -sh/MKK3 sublines were pre-treated (72 h) with doxycycline to induce sh/RNA expression, then exposed to either 5-FU (20 μM, 6 h), SB203580 (10 μM, 24 h) or their combination. Live cells were quantified by crystal violet staining 72 h after exposure. Viability was normalized to their respective sh/scr control set to 1.0. Means and Standard Deviations of results from two independent experiments are reported. Significance was assessed by two-tailed paired t test using Graph Pad Prism Software. **p < 0.01, ***p < 0.001