Fig. 1

Lung metastasis were reduced in C3aR deficiency breast tumor-bearing mice. a-f 4 T1 cells were orthotopically injected into WT (n = 7) or C3aR^−/− (n = 6) mice. Mice were anesthetized and sacrificed 28 days post tumor inoculation. a Lung metastases burden in the WT and C3aR^−/− tumor-bearing mice, and scans of H&E stained sections of the lungs of WT and C3aR^−/− mice with breast tumors. b Quantification of lung metastases. c-d Expression of E-Cadherin and Vimentin in the primary tumor tissue detected by immune-fluorescence, and western blot assay (e-f). (*P < 0.05, **P < 0.01, ***P < 0.001)