Fig. 6

Targeting C3aR Inhibits Lung Metastasis of Breast Cancer. a 4 T1 cells were inoculated into the third mammary fat pad of mice on day 0. C3aRA or PBS were injected i.p.10 mg/kg bodyweight from day 1, twice a week. b 28 days after tumor inoculation, lung metastasis burden in the C3aRA treated (n = 7) or PBS injected (n = 6) tumor-bearing mice. c-d Vimentin and E-Cadherin expression were detected by immunofluorescence assay. Data shown as three repeated experiments. e 4–5-week-old female MMTV-PyMT mice were intraperitoneally injected with (10 mg/Kg body weight) C3aR antagonist twice a week. f The experimental mice were euthanized at 16 weeks of age, the lungs were inflated with India ink, and the white nodules visible in the lungs represented the metastases burden. (*P < 0.05). g Kaplan–Meier curve for survival of human triple negative breast cancer patients from the TCGA-BRAC data sets. Data were obtained from the PROGgeneV2 web application and C3aR1 and PDGFA gene co-expression was divided into high and low at the median expression. n represents the number of patients at day 0. P-values less than 0.05 were considered significant