Fig. 1

SIRT7 overexpression is associated with poor prognosis in prostate cancer. a Meta-analysis of the SIRT7 expression in cancerous prostate tissues relative to that in normal prostate tissues retrieved from a prostate cancer dataset [23,24,25] in the Oncomine database. b Western blotting of SIRT7 expression in normal prostate epithelial and prostate cancer cell lines. c RT-PCR of SIRT7 expression in normal prostate epithelial and prostate cancer cell lines. d Representative IHC staining of SIRT7 on pairs of prostate tumor and normal adjacent tissues. Scale bar, 50 μm. e Analysis of mRNA level of SIRT7 in both prostate cancer and their adjacent normal tissues, retrieved from Wallace et al. [24]. f and g Kaplan-Meier overall survival and disease-free survival curves for all 572 patients with prostate cancer stratified by high and low SIRT7 expression. Each assay was performed in triplicate and the data are shown as the means ± SD. P-values were calculated by t-test (*P < 0.05; **P < 0.01; ***P < 0.001)