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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: SIRT7 depletion inhibits cell proliferation and androgen-induced autophagy by suppressing the AR signaling in prostate cancer

Fig. 5

SIRT7 upregulation in prostate cancer tissues correlates with the AR signaling (a) Analysis of the correlation between SIRT7 and AR mRNA expression in the prostate cancer dataset [25]. b Analysis of the association between SIRT7 and serum PSA expression in the prostate cancer dataset using the Oncomine database [48]. c Representative images of a human prostate cancer tissue array showing SIRT7 and AR protein levels in high-risk tumor and low-risk tumor. Scale bar, 50 μm. d Overall correlated expression of SIRT7 and AR protein in the human prostate cancer tissue array of (c). e Association between serum PSA and SIRT7 Allred score in each prostate cancer tissue specimen. f Immunofluorescence showed that SIRT7-depletion downregulated AR in prostate cancer cells. g Western blot results showing the modulation of the AR and its target genes in SIRT7-depleted prostate cancer cells. h RT-qPCR determined the modulation of the AR target genes in SIRT7-depleted prostate cancer cells. i Western blotting revealed that SIRT7 expression was not affected by the level of AR expression in prostate cancer cells. Each assay was performed in triplicate and the data are shown as the means ± SD. P-values were calculated by t-test or χ2-test (*P < 0.05; **P < 0.01; ***P < 0.001)

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