Fig. 3

Arnidiol induces mitochondrial fission in human breast cancer cells. a and b MDA-MB-231 cells were treated with Arn (60 μM) for 48 h, mitochondrial morphology was observed by MitoTracker Red CMXRos staining and confocal microscopy. Scale bars: 10 μm. Mitochondrial length was measured with ImageJ software. 50 cells from 3 independent experiments (mean ± SD, ^***P < 0.001 compared with control). c MDA-MB-231 cells were treated with various concentrations of Arn for 48 h or with Arn (60 μM) for different time intervals as indicated, the total cellular extracts were prepared and subjected to western blot using antibodies against Fis1, MFF, Mfn1, Mfn2 and OPA1. GAPDH was used as loading control