Fig. 6

A2aR knockdown outperforms an A2aR antagonist (SCH58261) on MSLN-CAR T cell functions in the presence of an A2aR agonist. Functional analysis of MSLN-CAR T cell including a proliferation, b cytokine production (IL-2, IFN-γ and TNF-α), and c cytotoxicity were performed in the presence of 1 μM A2aR antagonist (SCH58261) and 1 μM NECA. Data are representative of three independent experiments and are shown as mean ± SD. P < 0.05 were considered statistically significant. IL: interleukin; MSLN-CAR: fully human anti mesothelin CAR; NECA: 5′-(Nethylcarboxamido) adenosine; ns: Non-significant; SD: standard deviation. (**P < 0.01, ***P < 0.001)