Fig. 1
CLDN6 is directly upregulated by HIF-1α in breast cancer cells. a CLDN6 mRNA and protein levels under hypoxia in different molecular subtypes of breast cancer cells. b CLDN6 mRNA and protein levels after treatment with CoCl2 in different molecular subtypes of breast cancer cells. c WB analysis of HIF-1α and CLDN6 in MDA-MB-231 shNC or shHIF-1α cells under 1% O2 for 24 h. d Schematic diagram of the HRE sequences from the JASPAR database. e ChIP experiment to determine the combination of HIF-1α with the predicted HRE sequences in the CLDN6 promoter. * p < 0.05