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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: A SUMOylation-dependent HIF-1α/CLDN6 negative feedback mitigates hypoxia-induced breast cancer metastasis

Fig. 2

CLDN6 reduces breast cancer metastasis by inhibiting HIF-1α expression. a KEGG pathway analysis indicating that CLDN6 affects the HIF-1 signalling pathway. b WB analysis indicating that CLDN6 overexpression downregulates HIF-1α expression. c RT-PCR indicating that CLDN6 overexpression downregulates HIF-1α target gene expression. d WB analysis of EMT-related proteins in CLDN6-overexpressing and CLDN6/HIF-1α-overexpressing breast cancer cells under hypoxia for 24 h. e IF analysis of EMT-related proteins in CLDN6-overexpressing and CLDN6/ HIF-1α-overexpressing breast cancer cells. Scale: 100um. f WB analysis of HIF-1α and EMT-related proteins in CLDN6-KO HBL-100 cells. g Scratch and transwell invasion assays of CLDN6-overexpressing and CLDN6/HIF-1α-overexpressing breast cancer cells. Scale: 100um (above), 50um (below) * p < 0.05

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