Fig. 2From: A SUMOylation-dependent HIF-1α/CLDN6 negative feedback mitigates hypoxia-induced breast cancer metastasisCLDN6 reduces breast cancer metastasis by inhibiting HIF-1α expression. a KEGG pathway analysis indicating that CLDN6 affects the HIF-1 signalling pathway. b WB analysis indicating that CLDN6 overexpression downregulates HIF-1α expression. c RT-PCR indicating that CLDN6 overexpression downregulates HIF-1α target gene expression. d WB analysis of EMT-related proteins in CLDN6-overexpressing and CLDN6/HIF-1α-overexpressing breast cancer cells under hypoxia for 24 h. e IF analysis of EMT-related proteins in CLDN6-overexpressing and CLDN6/ HIF-1α-overexpressing breast cancer cells. Scale: 100um. f WB analysis of HIF-1α and EMT-related proteins in CLDN6-KO HBL-100 cells. g Scratch and transwell invasion assays of CLDN6-overexpressing and CLDN6/HIF-1α-overexpressing breast cancer cells. Scale: 100um (above), 50um (below) * p < 0.05Back to article page