Fig. 6

CLDN6 is lost in lymphatic metastasis and negatively associated with SENP1 and HIF-1α expression. a Immunohistochemical staining for CLDN6, SENP1 and HIF-1α expression in normal breast tissues. Scale: 20um. b Immunohistochemical staining for CLDN6, SENP1 and HIF-1α expression in breast cancer tissues. Scale: 20um. c Representative images showing CLDN6, SENP1 and HIF-1α staining in primary breast cancer tissues and matched lymphatic metastasis tissues. Scale: 200um (left), 20um (right). d CLDN6, SENP1 and HIF-1α IHC staining scores in primary breast cancer tissues (n = 50) and lymphatic metastasis tissues (n = 50) were shown. e Western blot of CLDN6, SENP1 and HIF-1α expression in random selected each 4 cases of normal breast tissues (N), breast cancer tissues (T) and lymph node metastasis (M). f Correlation between CLDN6 and HIF-1α protein expression in breast cancer tissues. g Correlation between CLDN6 and SENP1 protein expression in breast cancer tissues. h Correlation between SENP1 and HIF-1α protein expression in breast cancer tissues. * p < 0.05