Fig. 3

Cell death evaluation after PDT of AlPcS₄@NPs loaded MSCs in co-culture with Saos-2 cells. Graph representing quantification of total cell death (a) and survival rate (b), 24 h after PDT by Annexin V/PI and by Alamar Blue assay respectively, 5 × 10³ MSCs loaded with 90 μg/ml AlPcS₄@NPs were seeded into 24-well plate alone (grey bar) and in co-culture with 5 × 10³ or 15 × 10³ with Saos-2 cells (AlPcS₄@NPs@MSC:Saos-2; black bars) at different ratios (1:1 and 1:3 respectively). Graph representing quantification by flow cytometry of the percentage of live or dead cells for Saos-2 (dark grey bars) and AlPcS₄@NPs loaded MSCs (light gray bars) 24 h upon photoirradiation, 5 × 10³ MSCs loaded with 90 μg/ml AlPcS₄@NPs were seeded into 24-well plate in co-culture with Saos-2 cells at 1:1 and 1:3 ratios (5 × 10³ or 15 × 10³ cells respectively) (c). All data are expressed as mean ± SD (n = 3)