Fig. 2

Association analysis between urinary let-7c cluster and tumor stage. a Box-plot diagrams of urinary let-7c cluster stratified into pathological Ta and T1 stages. Relative expression levels were normalized to Unisp2 spike-in and calculated using the comparative ΔCt method. P-value were determined using the Mann-Whitney rank sum test. Boxes define the 25th and 75th percentiles; the horizontal lines into in the boxes indicates the median, and bars define the minimum and maximum values. b ROC curves plotted for diagnostic potential and discriminatory accuracy of urinary let-7c cluster to distinguish T1 from Ta stage. The corresponding Area Under the Curve (AUC) values are reported