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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Urinary expression of let-7c cluster as non-invasive tool to assess the risk of disease progression in patients with high grade non-muscle invasive bladder Cancer: a pilot study

Fig. 3

Analysis of let-7c miRNA cluster levels in BC cell lines. a Levels of let-7c cluster analyzed by qRT-PCR in intracellular (cells) and extracellular (culture medium) fractions of high grade (HG) NMIBC cells (RT-112). Relative expression was normalized with U6 snRNA and Unisp2 spike-in for intracellular and extracellular fraction respectively, and calculated using the comparative ∆Ct method. The extracellular values were normalized also to the cell count. N.D. indicates not determined miR-99a levels. b qRT-PCR of let-7c cluster of extracellular versus intracellular fraction (EC/IC ratio) in low grade (LG) NMIBC (RT-4), HG-NMIBC (RT-112) and HG-MIBC (T24) cells. Data are the average of three independent experiments; error bars indicate SEM. P-value was determined using the Mann-Whitney rank sum test

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