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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Molecular interplay between linc01134 and YY1 dictates hepatocellular carcinoma progression

Fig. 3

YY1 stimulates linc01134 transcription. a Databases predicted YY1 was the transcription factor of linc01134. b qRT-PCR analysis was used to determine the overexpression efficiency of YY1. c qRT-PCR detected the expression of linc01134 after YY1 overexpression. d qRT-PCR was used to determine knockdown efficiency of YY1. e qRT-PCR detected the expression of linc01134 after YY1 knockdown. f ChIP assay using anti-YY1 and anti-IgG was performed to explore the binding relation between YY1 and linc01134 promoter. g luciferase reporter assay detected luciferase activity of linc01134 promoter in transfected HEK-293 T cells. h Potential binding sites between YY1 with linc01134 promoter region were predicted. i ChIP assay measured the enrichment of linc01134 promoter region in anti-YY1 group. j Luciferase reporter assay measured luciferase activity of linc01134 promoter in transfected SNU-449 and HepG2 cells. **P < 0.01

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