Fig. 4

Linc01134 functions as ceRNA to up-regulate IGF2BP1 via sponging miR-324-5p. a MiRNAs that can bind to linc01134 were predicted by starBase and DIANA public databases. b qRT-PCR was detected the level of miRNAs after silencing linc01134. c The binding site between miR-324-5p and linc01134 was predicted by starBase. d Subcellular fractionation and FISH assays were conducted to understand the subcellular location of linc01134. e Luciferase reporter measured the luciferase activity of linc01134-WT/MUT under miR-324-5p mimics. f RNA pull down verified physical interaction between linc01134 and miR-324-5p. g qRT-PCR measured the expression of the indicated mRNAs after overexpressing miR-324-5p. h qRT-PCR assay was performed to examine the expression of IGF2BP1 in HCC tissues and para-tumor tissues. i Pearson correlation was performed to explore the correlation between linc01134 and IGF2BP1. j Potential binding site between miR-324-5p and IGF2BP1 was predicted by starBase. k-l Luciferase reporter and RIP verified the binding relation between IGF2BP1 and miR-324-5p. m The transfection efficiency of pcDNA3.1/linc01134 was detected by qRT-PCR. n RIP-based PCR evaluated the relative enrichment of linc01134 and IGF2BP1 in anti-Ago2 group. o qRT-PCR and western blot were conducted to measure the mRNA and protein levels of IGF2BP1 in transfected cells. *P < 0.05, **P < 0.01