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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Cullin-7 (CUL7) is overexpressed in glioma cells and promotes tumorigenesis via NF-κB activation

Fig. 3

CUL7 knockdown decreases invasive and migrative ability of glioma cells. a Representative images of invaded spheroids in 3D invasion assay for U87MG and U251 transfected with CUL7 and control siRNAs evaluated at 48 h and 96 h are shown (Scale bar = 200 μm). b Representative images of Transwell migration and invasion assays performed in U87MG and U251 transfected NC and si–CUL7 cells (Scale bar = 200 μm). c The area covered by invading cells quantitated after 96 h. d Graphic representation of migrated and invaded cells counts from Transwell assay. Data are represented as the mean ± SEM from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001, relative to control. e Western blot for protein levels of EMT components in lysates (20 μg) from U87MG and U251 cells transfected with siRNA against CUL7 and controls. GAPDH was used as a loading control. NC: non-silencing siRNA; si-CUL7: siRNAs targeting CUL7

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