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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Cullin-7 (CUL7) is overexpressed in glioma cells and promotes tumorigenesis via NF-κB activation

Fig. 4

CUL7 overexpression promotes proliferation, invasion and migration of glioma cells. U87MG and U251 cells transfected with GV141-CUL7s or Vectors and characterized in the following assays: (a, b) EdU performed 48 h after transfection (scale bar = 100 μm); (c) growth curve based on OD450 using the CCK-8 assay; (d, f) cell cycle profiles determined from PI staining in flow cytometry (e, g) % apoptosis as determined with Annexin V-FITC antibody and PI staining in flow cytometry. h Representative images of Transwell migration and invasion assays performed in U87MG and U251 cells. Graphic representation of migrated and invaded cells counts from Transwell assay. Data are represented as the mean ± SEM from three independent experiments (Scale bar = 200 μm). *P < 0.05; **P < 0.01; ***P < 0.001, relative to control. Vector: GV141-empty; CUL7: GV141-CUL7

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