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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Cullin-7 (CUL7) is overexpressed in glioma cells and promotes tumorigenesis via NF-κB activation

Fig. 7

CUL7 is a direct target of miR-3940-5p in glioma cells. a qRT-PCR analysis validated the lower expression of miR-3940-5p in glioma tissues. b Pearson r correlation was used to analyze the relationship between CUL7 and miR-3940-5p in Qilu hospital patient samples. (n = 14). c Overexpression of miR-3940-5p markedly suppressed the protein levels of CUL7 in glioma cells. d miR-3940-5p and its putative binding sequence in the wild-type (WT) and mutant (MUT) 3′-UTR of CUL7. e Overexpression of miR-3940-5p significantly decreased the luciferase activity that carried wild-type (WT) but not mutant type (MUT) 3′-UTR of CUL7 in glioma cells. f Western blot to detect expression levels of the MST1 and markers of activation of NF-κB pathway. GAPDH was used as a loading control. g Western blot for protein levels of cell cycle regulatory factors and EMT components in lysates (20 μg) from U87MG and U251 cells transfected with miR-3940-5p mimics and controls. GAPDH was used as a loading control. *P < 0.05; **P < 0.01; ***P < 0.001; NC: negative control RNA; si-CUL7: siRNAs targeting CUL7; miR-3940-5p: miR-3940-5p mimics

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